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Improve Your USP LC Gradient Separation Methods Following the New <621> Guidelines (HALO)
Guidelines for the use of UHPLC Instruments (Dr. Davy Guillarme, Prof. Jean-Luc Veuthey)
HPLC calculator (version 3.1) xlsx

The Chromatography and Sample Preparation Terminology Guide
Five Keys to Successful LC Methods
USP HPLC Packings
NIST SRM 870: Column Performance Test Mixture for Liquid Chromatography
A simple approach for reversed phase column comparisons via the Tanaka test


HPLC Column Selection - Are You Barking Up the Right Tree?
HPLC Column Classification - USP - Stimuli to the Revision Process
ACD Column Selector - the six chromatographic column parameters
SFC test for an extended ODS HPLC column classification (Article 1)
SFC test for an extended ODS HPLC column classification (Article 2)
SFC test for an extended ODS HPLC column classification (Article 3)
     This test provides, from a single analysis of carotenoid pigments, the absolute hydrophobicity, the silanol activity and the steric separation factor of the ODS stationary phases.


Useful links

HPLC Column Classification System (Katholieke Universiteit Leuven)
     More than 80 different types of ODS columns were characterized by 4 column parameters, which can be determined with 3 simple, fast, repeatable and reproducible chromatographic methods.
The Test Procedure (Katholieke Universiteit Leuven)

www.hplccolumns.org
http://www.hplccolumns.org/database/compare.php
     This website is a a free, online resource for selectivity data and tools for High Performance Liquid Chromatography. The current database contains column parameters for over 500 reversed-phase HPLC columns characterized using the Hydrophobic Subtraction Model of reversed-phase selectivity.

U.S. Pharmacopeia Column Equivalency Database
     The USP Database was developed by the USP Working Group on Column Equivalency using the NIST SRM 870. All data being displayed was generated by the column manufacturers after evaluation of the chromatographic phase.

ACD Column Selector (runs within the chemical drawing tool ACD ChemSketch Freeware)
     The columns are evaluated by 6 parameters (ligand density, hydrophobic sensitivity, shape selectivity, hydrogen bonding capacity, and total and acidic ion-exchange capacity). Since each parameter can be targeted individually, chromatographers can also search for columns that have high coefficients for a certain term, allowing them to change their columns to target a given structural aspect.

USP-NF Chromatographic Columns Online Database
The Chromatographic Columns online database is a compilation of the chromatographic columns used to validate the chromatographic procedures in USP–NF, Pharmacopeial Forum, Revision Bulletins, and Interim Revision Announcements. It provides a cumulative listing of columns referenced in gas and liquid chromatographic methods related to revisions made to USP–NF since January 1980.
Access
Chromatographic Columns, previously a print publication, is now a free, online database. To begin using the database, a one-time registration is required.  Also, after log in, Terms of Use must be agreed to before proceeding to the data.
     Register
     Login

www.chemicalize.org
This site was developed and is maintained by CHEMAXON (www.chemicalize.org) and contains most of the useful measurements that we need to help us understand analyte behaviour of a wealth of common, and some not so common, species.

MarvinSketch
A chemical structure drawing program containing some free plug-ins that can estimate/calculate physicochemical parameters of compounds.

Useful free tools for HPLC method development