HALO PAH, C30 phases

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ACE Method Development Kits

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33rd ISC (Budapest, Hungary)

21-25 September, 2020

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With ACE you hold all the aces ...

A new generation of ultra pure base deactivated columns

from Advanced Chromatography Technologies (ACT)

- Ultra high purity base deactivated silica
- 2um, 3um, 5um, 10um and 15um particle sizes
- Column diameters 0.3 .... 30mm
- Fully validated columns
- Exceptional reproducibility
- Excellent efficiency (up to 200,000 plates/metre)

ACE phases

Phase Functional group Endcapped Particle, um Pore, A Carbon, %
C4 C4 Yes 2, 3, 5, 10 100 5.5
C4-300 C4 Yes 3, 5, 10 300 2.6
C8 C8 Yes 2, 3, 5, 10 100 9.0
C8-300 C8 Yes 3, 5, 10 300 5.0
C18 C18 Yes 1.7, 2, 3, 5, 10 100 15.5
C18-HL (high load) C18 Yes 3, 5, 10, 15 90 20.0
AQ (aqueous) C18 Yes 2, 3, 5, 10 100 14.0
SuperC18 C18 Encapsulated 1.7, 2, 3, 5, 10 90 14.8
C18-Amide C18-Amide Yes 1.7, 2, 3, 5, 10 100 16.4
C18-AR C18-Phenyl Yes 1.7, 2, 3, 5, 10 100 15.5
C18-PFP (PentaFluoroPhenyl) C18-PF-Phenyl Yes 1.7, 2, 3, 5, 10 100 14.3
C18-300 C18 Yes 3, 5, 10 300 9.0
Phenyl Phenyl Yes 2, 3, 5, 10 100 9.5
Phenyl-300 Phenyl Yes 3, 5, 10 300 5.3
CN CN Yes 2, 3, 5, 10 100 5.5
CN-ES CN-alkyl Yes 1.7, 2, 3, 5, 10 100 12.6
NH2 NH2 No 1.7, 3, 5 100 4.0
CN-300 CN Yes 3, 5, 10 300 2.6
Silica Si --- 2, 3, 5, 10 100 ---
HILIC-A Si --- 1.7, 3, 5 100 ---
HILIC-B Aminopropyl --- 1.7, 3, 5 100 4.0
HILIC-N Polyhydroxy --- 1.7, 3, 5 100 7.0


Basic validation parameters (C18 column, 250 x 4.6mm x 5um)

    Specification Result
Peak Asymmetry - Amitriptyline % >90 97
  As0.1 <1.30 1.02
  USP Tailing Factor <1.20 1.05
Selectivity k’(Phenanthrene) 4.08 - 4.42 4.35
  k’(Desipramine) / k’(Phenanthrene) 0.120 - 0.130 0.125
  k’(Doxepin) / k’(Phenanthrene) 0.340 - 0.368 0.352
  k’(Imipramine) / k’(Phenanthrene) 0.496 - 0.538 0.517
  k’(Amitriptyline) / k’(Phenanthrene) 0.654 - 0.708 0.678

Mobile Phase: 80:20 MeOH / 25mM KH2PO4 (pH 6.0)
Flow Rate: 1.0ml/min, Temperature: 22°C, Wavelength: 215nm

Batch Validation Report, Test Chromatogram  see Downloads ... Misc.

Acidic-basic resistance

pH 1.8 Robustness
The high stability of ACE C18, C8, C4, CN and Phenyl phases was demonstrated: after three months usage at pH 1.8, no retention loss was observed with any ACE phase. Even cyano bonded columns (regarded to be most vulnerable to hydrolysis) showed excellent resistance.
pH 11.0 Robustness

Again, the ultra high purity ACE silica coupled with unique dense bonding technology effectively prevents silica dissolution. ACE columns withstand highly alkaline conditions without loss of column efficiency after 3 months usage at pH 11.0.


ACE capillary and nano columns

- High sensitivity
- Low sample mass and volume application
- Compatible with LC-MS and LC-MS/MS
- Very low solvent consumption
- Suitable for proteomics
- Compatible with LC-MS and LC-MS/MS


ACE UltraCore phases

Phase Functional group Endcapped Particle, um Pore, A Carbon, %
C18 C18 Encapsulated 2.5 / 5 95 7.0 / 5.4
Phenyl-Hexyl Phenyl-Hexyl Encapsulated 2.5 / 5 95 4.6 / 3.6








ACE HILIC UHPLC and HPLC Columns - Three HILIC column chemistries available; Method Development Kits for the same price as a single column

ACE HILIC Brochure

Step-by-Step Protocol for HILIC Method Development
ACE Step-by-Step HILIC Method Development Protocol (Flow Chart)
The Importance of Sample Diluent in HILIC
Column Equilibration in HILIC Mode
Selectivity Differences on the ACE HILIC Range
Comparing Selectivity in HILIC and RPLC Modes

A Simple, Generally Applicable HILIC Method Development Platform Based upon Selectivity
A Practical, Selectivity Based HILIC Method Development Protocol

Separation of Methylmalonic Acid and Succinic Acid using HILIC Mode
Separation of beta-Blockers using HILIC Mode
Separation of Caffeine and Related Compounds using HILIC Mode
Separation of Adenine and Nucleosides using HILIC-MS Mode
Separation of Nitrogenous Bases and Nucleosides using HILIC-MS Mode
Separation of Creatine and Metabolite Creatinine using HILIC Mode


ACE HPLC and UHPLC Columns Catalog
ACE HPLC and UHPLC Applications Guide
ACE UHPLC columns 1.7um
ACE HPLC Columns Catalog 2011/12
ACE HPLC Columns Catalog 2009/10


ACE Method Development Kits
ACE SuperC18 Brochure
ACE C18-Amide Brochure
ACE CN-ES Brochure
ACE Excel flyer
ACE Excel brochure
How to install ACE Excel UHPLC columns?
ACE UHPLC reusable column connector
ACE Generix Brochure
The Benefits of Ultra Inert Stationary Phases for the Reversed Phase HPLC of Biomolecules - Ranking of 300A Columns
ACE Capillary and Nano Columns, LC-MS and Rapid Analysis columns
ACE C18-PFP Brochure
ACE C18-AR Brochure
Improved HPLC Separation of Steroids
ACE Amino columns

UltraCore Columns

ACE UltraCore Brochure
ACE UltraCore SuperC18 MS Brochure
Buffer Recommendations for ACE UltraCore Columns

ACE Applications by Market Sector

Beauty applications
Clinical applications
Environmental applications
Food and beverage applications
Forensic applications
Mass spectrometry applications
Petrochemical applications
Pharmaceutical applications
USP monographs

Posters, Presentations

Exploring and Leveraging Multi-Mode Interactions to Maximise Chromatographic Selectivity with Uniquely Designed HPLC / UHPLC Stationary Phases
Exploring HPLC / UHPLC Chromatographic Selectivity with Low And High pH Eluents on a New Extended pH Range Stable Silica-Based Stationary Phase
Exploring Low and High pH Eluents Using a New Silica-Based Extended pH Range C18 Column for Analysis of Amphetamines and Opiates from Urine by LC-MS
Exploring the Power of Chromatographic Selectivity for Polar and Non-Polar Analytes with a Unique UHPLC / HPLC Polar Embedded Phase
Application of a Unique HPLC Phase when Exploring HPLC Method Development Challenges for a Pharmaceutical Combination Therapy Containing Five Active Ingredients
Low Level Determination of Synthetic Cannabinoids (SPICE) and Metabolites from Oral Fluid Using a Novel C18-Based Stationary Phase by UHPLC-MS/MS
Exploring the Selectivity and Performance of a New Extra Selectivity / Extended Stability Cyano Phase in UHPLC / HPLC Method Development
A New, Extended pH Range Stable Solid Core UHPLC / HPLC Column Family with SuperC18 and SuperPhenylHexyl Bonded Phases
Using Selectivity Data to Demonstrate a UHPLC / HPLC Method Development Platform Based upon Solid Core SuperC18 and SuperPhenylHexyl Phases
ACE C18-AR and C18-PFP: rational stationary phase design
Introducing reproducible selectivity changes to HPLC
Determination of Pesticides in Foodstuffs by LC-MS/MS (ACE UltraCore SuperC18)
Translating US Pharmacopoeia Methods to Sub-2 Micron and Solid Core Using the New USP <621> General Chapter Guidelines
Managing Method Transfer in the Pharmaceutical Laboratory
Method for the Determination of NDMA in Ranitidine Drug Substance and Drug Product (ACE C18-AR)


Buffer Selection Guide
Proteins and Peptides Purifications Guide
HPLC Troubleshooting Guide
C18 Comparison Guide
ACE Applications Guide
ACE Food and Beverage Applications Guide
ACE Clinical, Forensic and Bioanalysis Applications Guide
ACE Cannabis Applications Guide


ACE Stationary Phases
ACE Stationary Phases and Part Numbers
ACE Newsletter 1.
ACE Newsletter 2.
Reproducibility and Validation of ACE HPLC / UHPLC Columns
ACE Batch Validation Report
ACE Column Test Report
Solvent exchange between NP - RP phases
HPLC Column Regeneration
ACE Precolumn Filters
ACE Method Transfer Calculator (xls file)