HALO PAH, C30 phases

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ACE Method Development Kits

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33rd ISC (Budapest, Hungary)

21-25 September, 2020

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Superior products made by the

"silica boys"of EKA Chemicals

High pore volume and surface area and very high mechanical stability - even today this

combination of properties is unmatched by other chromatography packings on the market.

Kromasil and Kromasil Chiral are premium, silica-based HPLC packings, developed by Separation Products at Eka Chemicals. Reduced levels of Na, K, Ca, Fe, Al, and Mg make Kromasil packing materials useful for polar compounds which tend to interact with the silica surface. The wide range of particle sizes is an essential consideration for scale-up. The pH stability for bonded phases is 1.5 - 9.5

Kromasil phases

Phase Functional Group Endcapped Particle, um Pore, A
Silica - - 3.5, 5, 7, 10, 13, 16 60, 100, 300
C4 C4 Yes 1.8, 2.5, 3.5, 5, 7, 10, 13, 16 100, 300
C8 C8 Yes 1.8, 2.5, 3.5, 5, 7, 10, 13, 16 100, 300
C18 C18 Yes 1.8, 2.5, 3.5, 5, 7, 10, 13, 16 100, 300
Eternity C18 C18 Yes 2.5, 5 100
EternityXT C18 C18 Yes 1.8, 2.5, 5, 10 100
Eternity PhenylHexyl Phenyl / Hexyl Yes 2.5, 5 100
EternityXT PhenylHexyl Phenyl / Hexyl Yes 1.8, 2.5, 5, 10 100
Phenyl Phenyl Yes 5, 10, 16 100
NH2 NH2 Yes 5, 10 100
CN CN No 5, 10 60
DIOL DIOL No 5, 10 60
Chiral CHI-DMB - 5, 10, 16  
Chiral CHI-TBB - 5, 10, 16  
Chiral AmyCoat AmyCoat-RP 3, 5, 10, 25  
Chiral CelluCoat CelluCoat-RP 3, 5, 10, 25  



Kromasil columns and bulk phases

Kromasil 60A 1.8um 2.5um 3.5um 5um 7um 10um 13um 16um 25um
Kromasil 100A 1.8um 2.5um 3.5um 5um 7um 10um 13um 16um 25um
Eternity C18              
Eternity PhenylHexyl              
EternityXT PhenylHexyl          
Kromasil 300A 1.8um 2.5um 3.5um 5um 7um 10um 13um 16um 25um
Kromasil chiral 1.8um 2.5um 3um 5um 7um 10um 13um 16um 25um
Kromasil core-shell 1.8um 2.5um 3um 5um 7um 10um 13um 16um 25um

packed columns ONLY        columns and ALSO bulk sorbents

Bulk prep sorbents

Bulk sorbents are available in quantity of 200g, 500g, 1kg and 2kg.

Kromasil HPLC silica consists of perfectly spherical, totally porous particles. The uniqueness of Kromasil high performance spherical silica is the combination of:

- high surface area
- mechanical strength
- chemical purity
- chemical stability
- optimized surface properties
- well-defined pore structure


Kromasil chiral stationary phases are based on the well-known Kromasil premium spherical silica. The chiral polymer is covalently bonded to the functionalized Kromasil silica. Two phases have been developed to complement each other in selectivity:
Kromasil CHI-DMB:
The chiral monomer is 0,0'-bis (3,5-dimethylbenzoyl)-N,N'-diallyl-L-tartar diamide.
Kromasil CHI-TBB:
The chiral monomer is 0,0'-bis (4-tert-butylbenzoyl)-N,N'-diallyl-L-tartar diamide.

The new CelluCoat and AmyCoat chiral phases are based on a super-wide pore silica matrix developed in-house, and coated with a functionalized cellulose selector. High resolution, excellent selectivity, high pressure stability and stable performance when switching between compatible mobile phases are some important benefits. CelluCoat is equivalent to Chiralcel OD, AmyCoat is equivalent to Chiralpak AD of Daicel.

Test Chromatograms  see Downloads ... Chiral separations







Kromasil diC4 stationary phase for biomolecule separations
Kromasil C18(w): fully wettable HPLC phase for polar compounds
Eternity C18 Kromasil diC4 Brochure
Eternity C18 Kromasil C18(w) Brochure

Kromasil SFC columns

...... delivered in cyano, diol, silica, and 2-ethylpyridine chemistries
Eternity C18 Kromasil SFC Brochure

Kromasil Eternity long-life columns

Vary the pH from 1 to 12, repeatedly ... run at high temperatures, part of the time or all the time ....
Eternity C18 Kromasil Eternity Brochure
Eternity C18 Kromasil Eternity XT C18 10um Prep Brochure
Kromasil Eternity C18 and PhenylHexyl Columns: Presentation (pdf file)

Kromasil UHPLC columns

1.8um and 2.5um (100A) C4, C8 and C18 plases
Eternity PH Kromasil 1.8um UHPLC columns
Eternity PH Kromasil 2.5um UHPLC columns

Kromasil core-shell columns

Eternity C18 Kromasil ClassicShell Brochure
Eternity C18 Kromasil EternityShell Brochure

Catalogs, Brochures

Kromasil Classic Columns
Kromasil Product Catalogue
Kromasil Application Guide
Eternity PH Kromasil HILIC-D Brochure

Chiral Separations

Kromasil Chiral Brochure
Kromasil Chiral Stationary Phases
Kromasil AmyCoat and CelluCoat Chiral Stationary Phases Data Sheet
Kromasil AmyCoat and CelluCoat: New Polysaccharide-based Chiral Stationary Phases
Kromasil AmyCoat and CelluCoat Application Guide
Kromasil AmyCoat 3um: Rapid and Efficient Chiral Separations
Kromasil AmyCoat Test Chromatogram

Kromasil CelluCoat Test Chromatogram
Kromasil Chiral DMB and TBB
Kromasil Chiral DMB and TBB Application Guide
Chiral SFC Separations Using Different Polysaccharide Based Stationary Phases

 Peptides and Proteins Separations

Kromasil 300A: for your Protein Separations
RP Separation of Peptides and Proteins
HPLC Purification of Peptides (I)
HPLC Purification of Peptides (II)

Preparative Separations

Kromasil Preparative Sorbents
Loadability in Preparative Chromatography
Chiral Preparative Separations using Coated Polysaccharide Stationary Phases
Preparative Peptide Separations

 Posters, Presentations

Kromasil Eternity C18: Designed for Long Life
Kromasil Phenyl with High Ligand Coverage
Kromasil DIOL with High Ligand Coverage
RP Chromatography at Elevated pH (I)
RP Chromatography at elevated pH (II)
The Good RP HPLC Stationary Phases

Cross references to Kromasil products