Eprogen

 

News

New Core-Shell columns

01 July, 2014

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Promotion

Core-Shell columns: 399.99 EUR

until 31 December 2014

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Events

ISSS 2014 (Prague, Czech Republic)

30 Aug - 2 Sep, 2014

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think SMALL ... think FAST ... think NPS

The next generation of reverse phase protein and peptide columns.

Eliminating porosity can speed up HPLC analysis by as much as a factor of 10 while still maintaining high resolution and sensitivity.

Non porous phases

  Functional Group Particle, um Use
Columns for small molecule separations
NPS-Sil Si 1.5 These columns are highly efficient for all normal phase applications and work particularly well with very polar compounds. The surface silanols are only slightly acidic.
ODS-I C18 (polymeric) 1.5 Small drug molecules to biomolecules can be rapidly analyzed with ODS-I columns. With the addition of modifiers such as TEA, excellent peak shapes for many basic compounds such as tricyclic antidepressants can be obtained. This is an excellent column for high throughput and QC lab applications as well as LC/MS separations. The absence of porosity in NPS columns makes them suitable for the direct injection of plasma for metabolite analysis.
ODS-IIIE C18 (endcapped) 1.5 This is an excellent column for high throughput and QC lab applications, as well as LC/MS separations. The endcapping on ODS-IIIE columns delivers excellent resolution and analysis speed with low peak tailing for basic drug compounds.
NPS-PAH C18 (polymeric) 1.5 This PAH column offer high sensitivity, superior reproducibility, and the baseline separation of 16 PAH's in less than 10 minutes.
NPS-TAS C30 1.5 2,4-DNPH (dinitrophenylhydrazine) derivatives of aldehydes and ketones; vitamins and carotenoids in nutritional supplements.
Columns for proteins and peptides
ProteoSep HPRP C18 1.5 The HPRP column is designed specifically for proteins and peptides.  These analytes are usually found in very complex samples and in many cases are present with a numer of detergents, chaotropes and additives typical to protein and peptide solubilization. As such, special bonding chemistries are required to make the C-18 hydrophobic bonding extremely stable to these additives as well as accomodate the complex nature of proteins themselves. These surfaces are also required to withstand high pH (>9.5) samples and still retain longevity and resolving power. The HPRP column technology provides for this stability as well as the non porous nature of the support eliminating the need for extensive regeneration protocols required for all other silica based supports.

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Porous phases

  Functional Group Particle, um Use
SCD-100 C2 5 SCD columns are based on a short alkyl chain ligand bonded phase on 100A spherical silica with state- of-the-art base-deactivation.  Excellent peak shapes are obtained for many drug mixtures without the addition of silanol suppressing agents. 100% aqueous mobile phases can be routinely used. These columns are ideal for the analysis of basic and positively charged molecules, as well as neutral and acidic molecules.  They are especially useful for fast LC/MS analysis.
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RP-8 C8 6 RP-8 columns are monomerically bonded C8 chemistry on spherical 300A silica. 100% aqueous mobile phases can be routinely used. These columns are well suited for separating <150 kDa proteins and peptides, especially those of a more hydrophobic nature. The carbon chain length has little effect on selectivity in reversed phase chromatography of peptides and proteins.
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IEX phases (all columns are compatible with aqueous and most organic solvents in the pH range 2-8)

  Functional Group Particle, um Use
AX-300 week anion exchanger 6-7 A crosslinked polyethyleneimine phase on 300A silica. Selectivity can be altered by mobile phase composition; pH affects ionization of both the support and the solute. AX-300 permits analysis of proteins up to molecular weights of 200,000 daltons. This column has excellent recoveries and loading capacity: 22mg ovalbumin can be loaded onto a 250x4.6 column with no overloading effects.
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Q-300 strong anion exchanger 6-7 Fully quaternized, crosslinked polyethyleneimine phase on 300A silica. Ionization of this support has no pH dependence. Q-300 columns are excellent for the rapid separation of proteins and enzymes.
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CM-300 week cation exchanger 6-7 This support has a polyamide coating containing carboxymethyl groups bonded to a 300A silica. Selectivity can be altered by mobile phase salt composition, and pH affects ionization of both the support and the solute. CM-300 permits analysis of proteins up to molecular weights of 200,000 daltons and exhibits excellent recoveries as well as high loading capacity.
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S-300 strong cation exchanger 6-7 The support has a polyamide coating containing sulfopropyl functional groups bonded to a 300A silica. Ionization of the functional groups on this bonded phase is constant above pH 3.
S-300 columns are a good choice for the separation of basic proteins.
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S-1000 strong cation exchanger 7 The support has a polyamide coating containing sulfopropyl functional groups bonded to a 1000A silica. Ionization of only the solute is affected by pH above 3.
S-1000 columns are excellent for the fast analysis of glycosylated hemoglobin.
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GPC / SEC phases

  Particle, um Use
GPC-PEP 5 A glycerol bonded size exclusion support on a 50A spherical silica which can resolve small peptides (MW 0.8-30 kD). This support can resolve peptides which have at least a two-fold MW difference. Mobile phase optimization may be necessary due to the high variability in solubility, charge, and hydrophobicity of peptides. The exclusion limit for GPC Peptide makes it an effective "desalting column" for processing protein samples.
GPC-100 5 A glycerol bonded size exclusion support on a 100A spherical silica designed for the rapid analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers. This support is appropriate for globular molecules with MW ranges from 5-160 kD and linear molecules with MW ranges from 0.5-25 kD.
GPC-300 5 A glycerol bonded size exclusion support on a 300A spherical silica designed for the rapid analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers. This support is appropriate for globular molecules with MW ranges from 10-1000 kD and linear molecules with MW ranges from 2-100 kD.
GPC-500 7 A glycerol bonded size exclusion support on a 500A spherical silica designed for the rapid analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers. This support is appropriate for globular molecules with MW ranges from 40-2000 kD and linear molecules with MW ranges from 10-350 kD.
GPC-1000 A glycerol bonded size exclusion support on a 1000A spherical silica designed to effectively analyze polymers by size. This support has minimal interaction with anionic and neutral water-soluble polymers and is most frequently used with linear molecules possessing MW ranges from 40-1000 kD. It also allows for the separation of globular molecules such as protein multimers with MW ranges from 400-10,000 kD.
GPC-4000 10 A glycerol bonded size exclusion support on a 4000A spherical silica designed to analyze linear molecules with MW ranges from 70-10,000 kD.
CATSEC-100 5 Designed specifically for analysis of cationic polymers.  CATSEC-100 columns have a proprietary polymeric bonding chemistry on a 100A spherical silica support designed for separating linear molecules with molecular weight ranges of 0.5-25 kDa.  The polymerized coating enables polymers such as polyvinylpyridines to elute according to size and without adsorption.  Mobile phases are generally acidic and contain 0.1-0.2M salt to minimize adsorption and ion-exclusion.
CATSEC-300 5 Designed specifically for analysis of cationic polymers.  CATSEC-100 columns have a proprietary polymeric bonding chemistry on a 300A spherical silica support designed for separating linear molecules with molecular weight ranges of 2-100 kDa.  The polymerized coating enables polymers such as polyvinylpyridines to elute according to size and without adsorption.  Mobile phases are generally acidic and contain 0.1-0.2M salt to minimize adsorption and ion-exclusion.
CATSEC-1000 7 Designed specifically for analysis of cationic polymers.  CATSEC-100 columns have a proprietary polymeric bonding chemistry on a 1000A spherical silica support designed for separating linear molecules with molecular weight ranges of 40-1000 kDa.  The polymerized coating enables polymers such as polyvinylpyridines to elute according to size and without adsorption.  Mobile phases are generally acidic and contain 0.1-0.2M salt to minimize adsorption and ion-exclusion.

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