Chiral Technologies




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ACE Method Development Kits

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33rd ISC (Budapest, Hungary)

21-25 September, 2020

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...the market leader in enantioselective


Daicel and ChromTech columns: the most widely used and referenced chiral HPLC products worldwide

The most effective set of immobilized chiral columns, CHIRALPAK IA, IB, IC, and IG are used for primary screening. It is noteworthy that the four best columns are all meta-substituted.
The traditional CHIRALCEL and CHIRALPAK polysaccharide phases of Daicel consist of a derivatized cellulose or amylose physically coated onto a silica support. They are classified as Type II in the Wainer classification system and a wide range of column types are available. The ChromTech protein-based columns are suitable for all types of analytes - basic compounds can be separated on both CHIRAL-AGP and CHIRAL-CBH, acidic and neutral compounds can be separated on both CHIRAL-AGP and CHIRAL-HSA.

Primary Screening Columns
CHIRALPAK IA: Amylose tris (3,5-dimethylphenylcarbamate)
CHIRALPAK IB: Cellulose tris (3,5-dimethylphenylcarbamate)
CHIRALPAK IC: Cellulose tris (3,5-dichlorophenylcarbamate)
CHIRALPAK IG: Amylose tris (3-chloro-5-methylphenylcarbamate)


Application of polysaccharide phases

Name Structure Applications
OA -OCH3 small aliphatic compounds
CA-1 -OCH3 resolves racemates with azeotropic alcohols
OB -OPh small aliphatic and aromatic compounds
OC -ONHPh cyclopentenones
OK -OC=CPh aromatic compounds
OD -ONH3-5diMePh alkaloids, tropines, amines, beta blockers
OF -ONHpara-ClPh beta lactams, dihydroxypryidines, alkaloids
OG -ONHparaMePh beta lactams, alkaloids
OJ -OparaMePh aryl methyl esters, aryl methoxy esters
AD -ONH3-5diMePh alkaloids, tropines, amines, beta blockers
AS -ONH(CH3)CPh alkaloids, tropines, amines
WH and MA(+) ligand-exchange employed with an aqueous solution of copper sulfate (0.1 to 2mM)

most used


Cr(+) / Cr(-) Crown ether These columns contain a chiral crown ether as a chiral selector which is coated onto a 5um silica support.
Acidic mobile phases such as Perchloric acid pH 1 to 2, are used to operate these columns under standard conditions. Note that to shorten the retention time of hydrophobic samples, the addition of Methanol (15% maximum v/v) has been shown to be effective.
These columns are the reference columns for achieving amino acid separations, with the advantage that the elution order of the enantiomers can be reversed when necessary (CR(-) column gives the reversed elution order compared to CR(+) column).
Cr-I(+) / Cr-I(-) Crown ether These stationary phases are the newest addition to this family of chiral selectors. The crown ether selectors are immobilized on 5um silica support. Immobilization extends the range of solvents used for mobile phases such as acetonitrile and ethanol, thereby enhancing enantioselective resolution of chiral compounds. Use of the immobilized CROWNPAK CR-I(+) & CR-I(-) selectors also provides longer column life time.



Manuals, Data Sheets (pdf)

Chiralpak IA, IB, IC, ID, IE, IF and IG (Manual)
Chiralpak IA (DS)
Chiralpak IA (Manual)
Chiralpak IB (DS)
Chiralpak IB (Manual)
Chiralpak IC
Chiralpak ID
Chiralpak IE
Chiralpak IF
Chiralcel OA
Chiralcel OB and  OB-H
Chiralcel OC
Chiralcel OD
Chiralcel OD-H
Chiralcel OF
Chiralcel OG
Chiralcel OJ
Chiralcel OJ-H
Chiralcel OK
Chiralcel OZ
Chiralpak AD
Chiralpak AD-H
Chiralpak AS
Chiralpak AS-H
Chiralpak AY
Chiralpak AZ
Chiralpak QD-AX and QN-AX
Chiralpak ZWIX
Chiralpak AD-RH, AS-RH, Chiralcel OD-RH, OJ-RH


ChromTech CSPs

CHIRAL-AGP is most likely the column with the broadest applicability of all chiral columns available today. The column is used in the reversed-phase and can be used for direct resolution of enantiomers, without derivatization. The CHIRAL-AGP column separates enantiomers of an extremely broad range of drug compounds: amines (primary, secondary, tertiary, and quaternary), acids (strong and weak) and nonprotolytes (amides, esters, alcohols, sulphoxides, etc.). Very simple mobile phase systems are used for the majority of the separations - buffered waterbased mobile phases with the addition of low concentrations of an organic modifier, such as 2-propanol or acetonitrile. Most of the separations (>95%) have been performed using the above described simple mobile phases. However, in some cases charged modifiers as DMOA (N,N-dimethyloctylamine), octanoic acid and quaternary ammonium compounds are used to induce enantioselectivity.

CHIRAL-CBH is preferentially used for the separation of enantiomers of basic drugs from many compound classes. This chiral column separates compounds containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.). The mobile phases are mixtures of phosphate or acetate buffers and organic solvents as 2-propanol or acetonitrile containing 50mM disodium EDTA. The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and organic modifier.

CHIRAL-HSA is especially suited for the resolution of weak and strong acids, zwitterionic and nonprotolytic compounds. However, basic compounds have also been resolved. The column is operated in the reversed phase mode. Phosphate buffers with addition of organic modifiers are used as mobile phases. Retention and enantioselectivity can be regulated by changing the mobile phase composition. The addition of octanoic acid is necessary when chromatographing for example arylpropionic acid derivates on CHIRAL-HSA.

Fast Screening on Chiral AGP, CBH and HSA


Compound Type





Amine, hydrophobic





Amine, hydrophilic















Acid, weak





Acid, strong




 Ampholyte is a substance that can act as either an acid, in the presence of a strong base, or a base, when in the presence of a strong acid.

Mobile Phases

Group 1    AGP – 10mM (NH4 or Na)acetate buffer pH 4.5    CBH – 5% 2-propanol in 10mM Na-phosphate buffer pH 6.0

Groups 2, 3 and 5   ALL – 5% 2-propanol in 10mM Na-phosphate buffer pH 7.0

Groups 4 and 6    ALL – 10mM Na-phosphate buffer pH 7.0







CHIRALPAK IA-U and CHIRALPAK IC-U the first sub-2 micron chiral columns from Daicel
Sub-2 micron Chiral Columns from Daicel


Chiral Technologies Catalog
Product List and Catalog Numbers
ChromTech User's Guide


Chiralpak IA: the new Daicel chiral HPLC column compatible with all solvents
Chiralpak IA: Method Development
Chiralpak IB: Method Development
Chiralpak IA-IB-IC FAQs
Chiralpak AY-H and Chiralcel OZ-H: alternative selectivities
Chiralcel and Chiralpak FAQs
Chiralcel and Chiralpak Operating Conditions
Chiral Method Development Schemes
Chiralpak QD-AX/QN-AX for Acids and Amino Acid Derivatives
Chiral SFC columns
Use of Guard Cartridges for Chiralpak and Chiralcel Analytical Columns
Chiralpak ZWIX Brochure
Crownpak CR-I Applications

Posters, Presentations

Chiral Impurity Methods - Case Study
ChromTech Chiral AGP
ChromTech Chiral CBH

 ChromTech Application Bulletins

LC-MS Screening on Chiral AGP, HSA and CBH
Fast Screening on Chiral-AGP, Chiral-CBH and Chiral-HSA
Very Simple Method Development with Chiral-AGP
Method Optimization for LC-MS with Chiral-AGP
Strategies for Bioanalytical Work on Chiral-AGP
"Magic" Additives to Mobile Phases of Chiral-AGP
Coupling of Chiral-AGP and Chiral-CBH Columns for Optimum Resolution
Using Chiral-AGP and Chiral-CBH for LC-MS
Validated protein binding method used for more than 4000 drug compounds
Fast Gradient HPLC Method to Determine Compounds Binding to Human Serum Albumin
The Magic Tool for Method Development: Effect of pH


Useful links

Daicel online Applications Guide
Instruction Manuals

Online Guide to Chiral HPLC