Evosphere monodisperse HPLC particles

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HALO PCS: 3 for 2

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ISSS 2024 (Messina, Italy)

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Supplier of the world's largest range

of HPLC columns

Comprehensive batch validation ensures absolute batch-to-batch reproducibility

Combining an ultra pure silica with advanced bonding technology resulted in a densely bonded silica that is both highly robust and highly reproducible.

 Hichrom phases

Phase Functional group Endcapped Particle, um Pore, A Carbon, %
C8 C8 Yes 3.5, 5 150 8
C18 C18 Yes 3.5, 5 150 15
RPB C8-C18 Yes 3.5, 5, 10 110 14
PAH2 proprietary Yes 5 120 n/a
Chiral (D-Phenylglycine) CHIRA-chrom-1 No 5 n/a n/a
Chiral (L-Phenylglycine) CHIRA-chrom-1 No 5 n/a n/a
Chiral (DL-Phenylglycine) CHIRA-chrom-1 No 5 n/a n/a
Chiral (L-Leucine) CHIRA-chrom-1 No 5 n/a n/a
Chiral (Dinitrophenyltartramide) CHIRA-chrom-2 No 5 n/a n/a


C8 and C18 columns

Ultra pure porous silica
Advanced bonding technology
3.5um and 5um particle sizes
Column diameters 1.0 .... 21.2mm
Column lengths 10 .... 250mm
Improved acid / base stability
Excellent batch-to-batch reproducibility
High column efficiency
     3.5um: > 150,000 plates/metre
m: > 90,000 plates/metre

All columns are supplied with a Test Chromatogram and Batch Validation Certificate (Selectivity test report for acidic, basic and neutral molecules; Reproducibility test report for tricyclic antidepressants).
The report on the Test Chromatogram includes Efficiency data (N0.5) and two peak asymmetry calculations (BC0.1/AB0.1 and the much more rigorous N0.1/N0.5). Sample of the QC test mix is available on request.

Guard cartridge system GUARD CARTRIDGE SYSTEM
(JPEG file, opens in new window)


RPB (C8 / C18 multi-alkyl) columns (USP L42)

Designed for Reversed Phase separation of Basic materials
Ultra pure base deactivated porous silica
Exhaustive endcapping
Unique selectivity
3.5um, 5um, and 10um particle sizes
Column diameters 1.0 .... 21.2mm
Column lengths 10 .... 250mm
High column efficiency
     3.5um: > 145,000 plates/metre
     5um: > 90,000 plates/metre
m: > 50,000 plates/metre

PAH2 columns

Hichrom PAH2 is based on an alkyl bonded silica material with a high carbon loading, designed specifically for the analysis of polynuclear aromatic hydrocarbons.

Separation of 16 EPA PAHs on Hichrom PAH2 (JPEG file, opens in new window)


Chiral columns

Hichrom manufactures two ranges of Brush-type or "Pirkle" columns. These columns are designed to give a strong three-point interaction with one of an enantiomer pair. This means they are classified as Type I in the Wainer classification. There are two main types of stationary phases, pi-acceptor or pi-donor phases.
The Hichrom pi-acceptor phases (CHIRA-chrom-1 phases, USP L36) are 3,5-dinitrobenzoyl-derivatives of optically active amino acids, covalently bonded to aminopropyl silica. These columns are capable of separating a large range of compounds which includ
e a pi-donor aromatic group. This may be introduced by derivatization with naphthoyl chloride or other appropriate reagent.
The Hichrom pi-donor phase (CHIRA-chrom-2 phase) has a chiral dinitrophenyltartramide moiety bonded to the silica surface through a propyl spacer group. This phase requires the analyte to contain a pi-acceptor group such as the dinitrobenzoyl group. The dinitrobenzoyl group can easily be added to a wide range of compounds such as alcohols, amines, carboxylic acids etc. using dinitrobenzoyl chloride, isocyanate or dinitroaniline.

The advantages of the "Pirkle" type phases are that they are easily synthesized and are also readily available at reasonable cost. The disadvantages are that the columns will only work with aromatic compounds and that derivatization may need to be undertaken to aid the separation. It is important to note, however, that the derivatization is achiral and so does not present the problems involved in chiral derivatisation. Mobile phases are restricted to relatively non-polar organic solvents which is an advantage in preparative chromatography.

Enantiomer k' inversion

By substituting L-phenylglycine for D-phenylglycine, the order of elution of the chiral peaks can be reversed. Such a procedure can be useful in assigning peak identity or ensuring prior elution of the minor enantiomer, thus allowing its more accurate determination. The availability of the D,L-phenylglycine further aids peak assignment by removing chiral separations whilst maintaining background peak retention profile.

D-Phenylglycine column evaluation (JPEG file, opens in new window)