Hypersil

 

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25 January, 2014

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Trusted by scientists worldwide

Hypersil - one of the most robust, reproducible and reliable HPLC columns available

Whether you use classical Hypersil columns including ODS, Hypersil BDS base deactivated columns, or are looking for something new, we have a complete product line of HPLC columns to meet your needs.

Hypersil phases

Phase Functional group Endcapped Particle, um Pore, A
Silica - No 3, 5, 10 120
SAS C1 No 3, 5 120
HyPURITY C4 Yes 5 190
MOS C8 No 3, 5 120
MOS-2 C8 Yes 3, 5, 10 120
BDS C8 Yes 3, 5 130
HyPURITY C8 Yes 5 190
ADVANCE C8 Yes 3, 5 190
ODS C18 Yes 3, 5, 10 120
ODS-2 C18 Yes 5 80
BDS C18 Yes 3, 5 130
HyPURITY C18 Yes 3, 5 190
AQUASTAR C18 Yes 3, 5 190
Phenyl Phenyl No 3, 5, 10 120
Phenyl-2 Phenyl Yes 5, 10 120
BDS Phenyl Yes 3, 5 130
CPS CN No 3, 5, 10 120
CPS-2 CN Yes 5, 10 120
BDS CN Yes 3, 5 130
HyPURITY CN No 5 190
APS NH2 No 3, 5 120
APS-2 NH2 No 3, 5 120
         
Hypercarb     3, 5, 7 250

 

Silica
Hypersil Silica is the robust and reproducible base material for all of the high quality classical Hypersil bonded phases.  Unbonded, it is an efficient and selective tool for chromatography of non-polar and moderately polar organic compounds by normal phase chromatography.

SAS
Hypersil SAS has as a trimethyl group (C1 alkyl chain) stationary phase to provide the shortest retention in comparison to other alkyl-modified phases for non-polar compounds. Hypersil SAS has shown unique selectivity for polar analytes and multi-functional compounds and is excellent at ion pair chromatography. For new method development, we recommend the use of one of newer BetaBasic 4 or HyPURITY C4 columns.

HyPURITY C4
HyPURITY C4 columns are based on the unique HyPURITY silica, designed to be essentially metal free and exceptionally stable at low pH.  The HyPURITY silica manufacturing process ensures reproducibility and quality, and the homogeneous surface leads to uniform bonding coverage and reduced silanol-analyte interactions that result in poor peak shape for bases, acids and chelating compounds.  The 190Å pore size and excellent mass transfer properties of HyPURITY packings make them useful for the chromatography of large peptides as well as small molecules, with unbeatable peak shape for all sample types.  HyPURITY C4 columns are recommended for very hydrophobic compounds that have too long elution times when injected onto a C18 or C8 column.

MOS
Hypersil MOS is a dimethyloctylsilane (C8 alkyl chain) non-endcapped stationary phase. The C8 column uses the same silica Hypersil ODS, consequently the selectivity is similar to that of Hypersil ODS columns, but the lower carbon content makes them less retentive, giving faster analysis times. Hypersil MOS is suitable for non-polar to moderately polar compounds, but is not recommended for basic compounds. HyPURITY C8 or BetaBasic 8 columns are recommended for new method development and the analysis of basic compounds.

MOS-2
Hypersil MOS-2 is a dimethyloctylsilane (C8 alkyl chain) fully endcapped stationary phase. The columns are based  on the same silica as the Hypersil ODS phase, so they have similar selectivity to Hypersil ODS columns but the lower carbon content makes them less retentive. Hypersil MOS-2 columns are suitable for non-polar to moderately polar compounds, but are not recommended for basic compounds. HyPURITY C8 or BetaBasic 8 columns are recommended for new method development and the analysis of basic compounds.

BDS-C8
Hypersil BDS receives a special base deactivation treatment during silica material manufacture to reduce silanophilic activity. Hypersil BDS C8 is endcapped and suitable for chromatography of basic as well as acidic and neutral compounds. Hypersil BDS has an excellent reproducibility record, with data available for column to column and batch to batch to prove its suitability for validated methods. Hypersil BDS phases are ideal for the determination of pharmaceutical active components.  For new methods development or the determination of basic compounds we recommend our latest silica phases such as HyPURITY C8 or BetaBasic 8 columns.

HyPURITY C8
HyPURITY C8 columns are based on the unique HyPURITY silica, designed to be essentially metal free and exceptionally stable at both high and low pH.  The HyPURITY silica manufacturing process ensures reproducibility and quality, and the homogeneous surface leads to uniform bonding coverage and reduced silanol-analyte interactions that result in poor peak shape for bases, acids and chelating compounds.  The 190Å pore size and excellent mass transfer properties of HyPURITY C8 columns make them useful for the chromatography of large peptides as well as small molecules, with unbeatable peak shape for all sample types.  HyPURITY C8 columns arerecommended when HyPURITY C18 columns provide too much retention, and are proven  for the separation of peptides and components of tryptic digests.

HyPURITY ADVANCE C8
HyPURITY ADVANCE contains a polar amide group embedded within a C8 chain, and is fully endcapped. The columns are based on our metal free HyPURITY silica and, compared to other phases, produces a competely new selectivity. A polar functional group between alkyl ligands and the silica surface allows faster analysis without compromising the resolving power. The phase chemistry allows three interaction mechanisms with samples:
The polar character assists retention of polar compounds and limits interactions of samples with the silica surface - which reduces retention of basic compounds.
This effect often results in a completely new elution order when compared to traditional alkyl bases chemistries, and gives the columns complete stability in 100% aqueous mobile phases. 
The C8 ligand gives moderate retention for neutral samples, hence HyPURITRY ADVANCE is ideal for high throughoput laboratories with complex and variable sample types.

ODS
Hypersil ODS columns from Thermo Hypersil-Keystone are well established and referenced in many HPLC methods as an excellent C18 phase for a broad range of applications.  The material is endcapped and is available in 3, 5 and 10µm particle sizes. Hypersil ODS columns are suitable for non-polar and moderately polar analytes including acids, neutrals and lipophilic compounds.  For basic compounds and new method development, we recommend BetaBasic 18 or HyPURITY C18 columns.

ODS-2
Hypersil ODS-2 columns are well tested and quality checked equivalents to Waters Spherisorb ODS-2 columns. As an inexpensive alternative this phase provides the same hydrophobic and ion exchange behavior and selectivity. Hypersil ODS-2 has been subjected to a wide range of quality control tests to confirm the direct comparison between the two materials.

BDS-C18
Hypersil BDS C18 receives a special base deactivation treatment during silica material manufacture to reduce silanophilic activity. Hypersil BDS C18 is endcapped and suitable for chromatography of basic compounds. Hypersil BDS has an excellent reproducibility record, with data available for column to column and batch to batch to prove its suitability for validated methods. Hypersil BDS phases are ideal for the determination of pharmaceutical active components, however for new methods we recommend our latest HyPURITY C18 or BetaBasic 18 columns.

HyPURITY C18
HyPURITY C18 columns are based on the latest in silica technology - HyPURITY silica, which has less than 10ppm total metals and is exceptionally stable at low pH.  The HyPURITY silica manufacturing process ensures reproducibility and quality. The homogeneous surface leads to uniform bonding coverage and eliminates silanol-analyte interactions that cause poor peak shapes for bases, acids and chelating compounds.
HyPURITY C18 columns undergo forty separate quality control tests to meet stringent performance specifications. The 190Å pore size and excellent mass transfer properties of HyPURITY C18 makes it ideal for chromatography of large peptides, as well as small molecules, with unbeatable peak shape for all sample types.  HyPURITY columns have proven performance for a wide range of applications, including small pharmaceuticals and peptides.

HyPURITY AQUASTAR C18
A new member of the HyPURITY family introduced at Pittcon 2003, HyPURITY AQUASTAR columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. The robust nature of the bonded phase results in a silica-based column that is unsurpassed for LC and LC/MS applications. This next generation column also offers enhanced performance over first generation polar end-capped columns.
HyPURITY AQUASTAR columns combine a C18 ligand with polar end-capping to produce a highly stable material for reversed phase chromatography.  HyPURITY AQUASTAR columns have the unique ability to retain and separate polar analytes in 100% aqueous conditions, with no phase collapse. Selectivity and sensitivity can be maintained at decreased levels of buffer concentrations, making HyPURITY AQUASTAR ideal for applications involving MS detection.

PHENYL
Hypersil Phenyl is a non endcapped stationary phase, and has unique properties for analysis of aromatic compounds and moderately polar compounds. The retention characteristics are similar to those of a C8 (MOS) stationary phase, however the selectivity is different, making this phase ideal for aromatic compounds and polycyclic aromatic hydrocarbons. Hypersil Phenyl columns offer 'alternative' selectivity compared to alkyl chain phases due to pi-pi interactions from the benzene ring, which makes them a good choice for compounds typically considered difficult to analyze. The phenyl modification also permits the use of the columns under normal phase LC conditions.

PHENYL-2
Hypersil Phenyl-2 is an endcapped stationary phase, and has unique properties for analysis of aromatic compounds and moderately polar compounds. The retention characteristics are similar to those of a C8 (MOS-2) stationary phase, however the selectivity is different, making this phase ideal for aromatic compounds and polycyclic aromatic hydrocarbons. Hypersil Phenyl-2 columns offer 'alternative' selectivity compared to alkyl chain phases due to pi-pi interactions from the benzene ring, which makes them a good choice for compounds typically considered difficult to analyze. The phenyl modification also permits the use of the columns under normal phase LC conditions.

BDS-PHENYL
Hypersil BDS receives a special base deactivation treatment during silica material manufacture to reduce silanophilic activity. Hypersil BDS Phenyl is endcapped and suitable for chromatography of basic compounds. Hypersil BDS has an excellent reproducibility record, with data available for column to column and batch to batch to prove its suitability for validated methods.  All BDS phases are generally suitable for reversed phase chromatography, however the phenyl columns are also suitable for normal phase chromatography.  For the determination of very basic aromatic compounds we recommend our BetaBasic Phenyl columns.

CPS
Hypersil CPS columns can be used in both reversed phase and normal phase chromatography. Under normal phase conditions these materials provide a different selectivity to the Hypersil Silica and APS-2 phases. The cyano stationary phase is not deactivated by traces of water and can be used with gradient mobile phases in normal phase chromatography. In reversed phase LC Hypersil CPS columns provide an alternative selectivity to the alkyl-chain silicas, with lower hydrophobicity. Please note that all CPS columns are shipped in normal phase solvents unless otherwise requested.

CPS-2
Hypersil CPS-2 columns can be used in both reversed phase and normal phase chromatography. Under normal phase conditions these materials provide a different selectivity to the Hypersil Silica and APS-2 phases. The cyano stationary phase is not deactivated by traces of water and can be used with gradient mobile phases in normal phase chromatography. In reversed phase LC Hypersil CPS-2 columns provide an alternative selectivity to the alkyl-chain silicas, with lower hydrophobicity. Due to the presence of an end-capping molecule they show slightly different selectivity to the non-endcapped Hypersil CPS column. Please note that all CPS-2 columns are shipped in normal phase solvents unless otherwise requested.

BDS-CN
Hypersil BDS receives a special base deactivation treatment during silica material manufacture to reduce silanophilic activity. Hypersil BDS Cyano is endcapped and suitable for chromatography of basic compounds. Hypersil BDS has an excellent reproducibility record, with data available for column to column and batch to batch to prove its suitability for validated methods. Hypersil BDS Cyano columns are suitable for reversed phase or normal phase chromatography. For new methods development and the determination of basic compounds we recommend our BetaBasic Cyano columns.

HyPURITY CN
HyPURITY Cyano columns are based on the unique HyPURITY silica, designed to be essentially metal free and exceptionally stable at low pH.  The HyPURITY silica manufacturing process ensures reproducibility and quality, and the homogeneous surface leads to uniform bonding coverage and reduced silanol-analyte interactions that result in poor peak shape for bases, acids and chelating compounds.  The 190Å pore size and excellent mass transfer properties of HyPURITY packings make them useful for the chromatography of large peptides as well as small molecules, with unbeatable peak shape for all sample types.  HyPURITY columns have proven performance for the separation of peptides and components of tryptic digests.  HyPURITY Cyano columns can be used in both reversed phase and normal phase separations.  In normal phase, HyPURITY Cyano columns offer alternative selectivity to silica phases. In reversed-phase, HyPURITY Cyano columns offer alternative and faster selectivity than many alkyl type phases (C18/C8).

APS
Hypersil APS columns are a versatile aminopropyl group stationary phase which can be used in 3 modes: ion chromatography (weak anion exchanger), reversed phase and normal phase chromatography. Hypersil APS columns can be used with traditional buffers and organic additives as a weak anion exchanger for the analysis of anions and organic acids. In reversed phase mode, Hypersil APS columns are ideal for carbohydrate analysis.  They also offer an alternative selectivity to silica when used in normal phase.  Please note that all Hypersil APS columns are shipped in normal phase solvents unless otherwise requested.

APS-2
Hypersil APS-2 columns are a versatile aminopropyl group stationary phase which can be used in 3 modes: ion chromatography (weak anion exchanger), reversed phase and normal phase chromatography. Hypersil APS-2 columns can be used with traditional buffers and organic additives as a weak anion exchanger for the analysis of anions and organic acids. In reversed phase mode, Hypersil APS-2 columns are ideal for carbohydrate analysis.  They also offer an alternative selectivity to silica when used in normal phase.  Please note that all Hypersil APS-2 columns are shipped in normal phase solvents unless otherwise requested.

Hypercarb
Hypercarb is a unique material for HPLC manufactured by Thermo Hypersil-Keystone. Hypercarb consists of fully porous spherical carbon particles comprised of flat sheets of hexagonally arranged carbon atoms. The carbon atoms have a fully satisfied valence and offer completely different retention and selectivity to silica and polymer based phases.
Hypercarb can be used for RP and NP chromatography with a pH range from 1 to 14, and at extremes of temperature. Hypercarb excels in the analysis of structurally related components (e.g. stereo- and diastereoisomers and positional isomers) where no separation occurs on conventional materials.
Hypercarb retains polar compounds via a 'polar retention effect' where a stronger analyte-sorbent attraction occurs as the polarity of the analyte increases, so the more polar the analyte the longer it's retention time compared to silica-based phases. Due to the unique retention mechanism of Hypercarb, we recommend that you do not use a column longer than 100mm or 150mm in length.